關(guān)于森貝伽
森貝伽生物依托國(guó)內(nèi)外技術(shù)研發(fā)人才的優(yōu)勢(shì),由留美資深科學(xué)家與數(shù)位國(guó)內(nèi)多年從事生物領(lǐng)域研究的博士、碩士等高校生物領(lǐng)域研究人員共同創(chuàng)立,在科學(xué)研究和醫(yī)療檢測(cè)領(lǐng)域享有極高的聲譽(yù)。
elisa試劑盒-森貝伽自主品牌
擁有自己的實(shí)驗(yàn)室與研發(fā)基地,專(zhuān)業(yè)的技術(shù)研發(fā)團(tuán)隊(duì), 長(zhǎng)期致力于ELISA試劑盒、生物試劑的研發(fā),擁有10年研發(fā)經(jīng)驗(yàn)。檢測(cè)目的:
用于測(cè)定血清,血漿及相關(guān)液體等樣本。例如適合檢測(cè)包括血清、血漿、尿液、胸腹水、灌洗液、腦脊液、細(xì)胞培養(yǎng)上清、組織勻漿等標(biāo)本。森貝伽供應(yīng)的種屬:人、大鼠、小鼠、豚鼠、兔子、豬犬、牛羊、雞鴨、植物ELISA試劑盒等售后技術(shù):公司提供全方位的售前、售中、售后技術(shù)支持,為您解決實(shí)驗(yàn)過(guò)程中遇到的問(wèn)題客戶(hù)案例:憑借著森貝伽實(shí)驗(yàn)室先進(jìn)的技術(shù)平臺(tái)和專(zhuān)業(yè)的售后技術(shù)服務(wù),此期間我們已為全國(guó)眾多醫(yī)療機(jī)構(gòu)、重點(diǎn)高校實(shí)驗(yàn)室及研究所提供了ELISA產(chǎn)品及ELISA代測(cè)服務(wù),深受各高校實(shí)驗(yàn)室和醫(yī)院的師生歡迎。需要elisa實(shí)驗(yàn)代做的請(qǐng)點(diǎn)擊進(jìn)入elisa代測(cè)技術(shù)服務(wù)
——以下是部分SCI引用案例——
1.文章題目:An inhibitor of the Keap1-Nrf2 protein-protein interaction protects NCM460 colonic cells and alleviates experimental colitis.
發(fā)表時(shí)間:24 May 2016
作 者:Meng-Chen Lu, Jian-Ai Ji, Yong-Lin Jiang, Zhi-Yun Chen, Zhen-Wei Yuan, Qi-Dong You & Zheng-Yu Jiang
作者單位:Department of Medicinal Chemistry, School of Pharmacy, China Pharmaceutical University,中國(guó)藥科大學(xué)藥學(xué)院藥物化學(xué)實(shí)驗(yàn)室
使用產(chǎn)品:IL-1β, IL-6, TNF-α and IFN-γ Production. Levels of IL-6 (IL-6 (m) ELISA kit
https://www.nature.com/articles/srep26585.pdf
2.文章題目:Metabolic and microbial signatures in rat hepatocellular carcinoma treated with caffeic acid and chlorogenic acid
發(fā)表時(shí)間:03 July 2017
作 者:Zhan Zhang, Di Wang, Shanlei Qiao, Xinyue Wu, Shuyuan Cao, Li Wang, Xiaojian Su & Lei Li
作者單位:Department of Hygiene Analysis and Detection, School of Public Health, Nanjing Medical University,南京醫(yī)科大學(xué)公共衛(wèi)生學(xué)院
使用產(chǎn)品:rat LPS ELISA kit
https://www.nature.com/articles/s41598-017-04888-y.pdf
3.文章題目:p-Hydroxylcinnamaldehyde induces the differentiation of oesophageal carcinoma cells via the cAMP-RhoA-MAPK signalling pathway
發(fā)表時(shí)間:09 August 2016
作 者:Ming Ma, Lian-mei Zhao, Xing-xiao Yang, Ya-nan Shan, Wen-xuan Cui, Liang Chen & Bao-en Shan
作者單位:Clinical Laboratory, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P. R. China,河北醫(yī)科大學(xué)第四醫(yī)院檢驗(yàn)科
使用產(chǎn)品: cAMP, CEA and SCC ELISA kit
https://www.nature.com/articles/srep31315.pdf
4.文章題目:A preliminary study of ALPPS procedure in a rat model
發(fā)表時(shí)間:03 December 2015
作 者:Huawen Shi, Guangchao Yang, Tongsen Zheng, Jiabei Wang, Lulu Li, Yingjian Liang, Changming Xie, Dalong Yin, Boshi Sun, Jing Sun, Huanlai Wang, Shangha Pan, Hongchi Jiang, WanYee Lau & Lianxin Liu
作者單位:Key Laboratory of Hepatosplenic Surgery, Ministry of Education, Department of General Surgery, the First Affiliated Hospital of Harbin Medical University, Harbin, China 哈爾濱醫(yī)科大學(xué)肝脾外科省部共建教育部重點(diǎn)實(shí)驗(yàn)室
使用產(chǎn)品: NF-KB ELISA kit
https://www.nature.com/articles/srep17567.pdf
5.文章題目:In vitro assessment of the glucose-lowering effects of berberrubine-9-O-β-D-glucuronide, an active metabolite of berberrubine
發(fā)表時(shí)間:02 January 2017
作 者:Na Yang, Run-bin Sun, Xing-long Chen, Le Zhen, Chun Ge, Yu-qing Zhao, Jun He, Jian-liang Geng, Jia-hua Guo, Xiao-yi Yu, Fei Fei, Si-qi Feng, Xuan-xuan Zhu, Hong-bo Wang, Feng-hua Fu, Ji-ye Aa & Guang-ji Wang
作者單位:
Key Lab of Drug Metabolism and Pharmacokinetics, Jiangsu Key Laboratory of Drug Design and Optimization, China Pharmaceutical University, Nanjing 210009, China
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
Department of Pharmacology, Clinical Research Institute of Jiangsu Provincial Hospital of Traditional Chinese Medicine, Nanjing 210009, China
Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Pharmacy School at Yantai University, Yantai 264005, China
使用產(chǎn)品: mouse α-glucosidase ELISA kit
https://www.nature.com/articles/aps2016120.pdf
6.文章題目:DT-13 ameliorates TNF-α-induced nitric oxide production in the endothelium in vivo and in vitro
發(fā)表時(shí)間:21 November 2017
作 者:RuipingFana1 YuweiHanab1 HanHana Zhengdong Chena Boyang YuaJunping Koua Yuanyuan Zhanga
作者單位:
a Jiangsu Key Laboratory of TCM Evaluation and Translational Research, Department of Complex Prescription of TCM, China Pharmaceutical University, 639
Longmian Road, Nanjing 211198, China
b Institute of Neurology, General Hospital of Shenyang Military Command, Shenyang, Liaoning, 110016, China
使用產(chǎn)品: Mouse nitric oxide ELISA kit,IL-8 ELISA kit, MCP-1 ELISA kit, ROS ELISA kit, TNFR ELISA kit
https://www.sciencedirect.com/science/article/pii/S0006291X17321824
——以上是部分SCI引用案例——
森貝伽ELISA試劑盒組成:
產(chǎn)品組成 | 48孔配置 | 96孔配置 | Storage |
標(biāo)準(zhǔn)品:180ng/L | 0.5ml×1瓶 | 0.5ml×1瓶 | 2-8℃ |
標(biāo)準(zhǔn)品稀釋液 | 1.5ml×1瓶 | 1.5ml×1瓶 | 2-8℃ |
酶標(biāo)試劑 | 3ml×1瓶 | 6ml×1瓶 | 2-8℃ |
樣品稀釋液 | 3ml×1瓶 | 6ml×1瓶 | 2-8℃ |
顯色劑A液 | 3ml×1瓶 | 6ml×1瓶 | 2-8℃ |
顯色劑B液 | 3ml×1瓶 | 6ml×1瓶 | 2-8℃ |
終止液 | 3ml×1瓶 | 6ml×1瓶 | 2-8℃ |
濃縮洗滌液 | (20ml×20倍)×1瓶 | (20ml×30倍)×1瓶 | 2-8℃ |
酶標(biāo)包被板 | 1×48 | 1×96 | 2-8℃ |
封板膜 | 2片 | 2片 | RT |
密封袋 | 1個(gè) | 1個(gè) | RT |
說(shuō)明書(shū) | 1份 | 1份 | RT |
操作步驟: 注意事項(xiàng):1.試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開(kāi)封后如未用完,板條應(yīng)裝入密封袋中保存。2.濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果。3.各步加樣均應(yīng)使用加樣器,并經(jīng)常校對(duì)其準(zhǔn)確性,以避免試驗(yàn)誤差。一次加樣時(shí)間最好控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排槍加樣。4.請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線,最好做復(fù)孔。如標(biāo)本中待測(cè)物質(zhì)含量過(guò)高(樣本OD值大于標(biāo)準(zhǔn)品孔第一孔的OD值),請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)(n倍)后再測(cè)定,計(jì)算時(shí)請(qǐng)最后乘以總稀釋倍數(shù)(×n×5)。5.封板膜只限一次性使用,以避免交叉污染。6.底物請(qǐng)避光保存。7.嚴(yán)格按照說(shuō)明書(shū)的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).8.所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理。9.本試劑不同批號(hào)組分不得混用。10.如與英文說(shuō)明書(shū)有異,以英文說(shuō)明書(shū)為準(zhǔn)。標(biāo)準(zhǔn)曲線的繪制方法:
可以采用各種繪圖軟件來(lái)繪制ELISA標(biāo)準(zhǔn)曲線,我公司以“Curve Exert1.3”軟件為例,繪制的ELISA標(biāo)準(zhǔn)曲線如下:計(jì)算:以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的OD值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度;再乘以稀釋倍數(shù);或用標(biāo)準(zhǔn)物的濃度與OD值計(jì)算出標(biāo)準(zhǔn)曲線的直線回歸方程式,將樣品的OD值代入方程式,計(jì)算出樣品濃度,再乘以稀釋倍數(shù),即為樣品的實(shí)際濃度