一.人N端中段骨鈣素(N-MID-OT)ELISA試劑盒詳細(xì)介紹產(chǎn)品貨號(hào):SBJ-H1098
中文名稱:人N端中段骨鈣素(N-MID-OT)ELISA試劑盒
英文名稱:Human N-MID Osteocalcin,N-MID-OT ELISA kit
規(guī)格: 96T/48T
品牌:森貝伽
英文縮寫:N-MID-OT
種屬:人ELISA試劑盒
檢測波長:450 nm
所需樣本體積: 50-100ul
適用范圍:僅供科研
保存及有效期:2-8℃,六個(gè)月
檢測目的:用于測定血清,血漿及相關(guān)液體等樣本。例如適合檢測包括血清、血漿、尿液、胸腹水、灌洗液、腦脊液、細(xì)胞培養(yǎng)上清、組織勻漿等標(biāo)本。
森貝伽供應(yīng)的種屬有:人、大鼠、小鼠、豚鼠、兔子、豬犬、牛羊、雞鴨、植物ELISA試劑盒等
二.訂貨信息: 人N端中段骨鈣素(N-MID-OT)ELISA試劑盒 貨號(hào) | 名稱 | 規(guī)格 | 品牌 | 檢測方法 | 貨期 | 價(jià)格 |
SBJ-H1098 | 人N端中段骨鈣素(N-MID-OT)ELISA試劑盒 | 96T/48T | 森貝伽 | ELISA | 現(xiàn)貨 | 點(diǎn)擊訂購 |
【獨(dú)特優(yōu)勢】
1、產(chǎn)品種類齊全、質(zhì)量可靠、價(jià)格優(yōu)惠、靈敏度高、效果穩(wěn)定、易保存、操作簡便
2、免費(fèi)提供產(chǎn)品最新報(bào)價(jià)、實(shí)驗(yàn)原理、產(chǎn)品用途及中英文說明書
3、發(fā)貨及時(shí)(南京本地客戶有專門人員送貨上門及取標(biāo)本)
4、免費(fèi)提供ELISA代測服務(wù),森貝伽擁有自己的實(shí)驗(yàn)室(北京、南京、上海、武漢)和技術(shù)團(tuán)隊(duì),公司提供全方位的售前、售中、售后技術(shù)支持,為您解決實(shí)驗(yàn)過程中遇到的問題。想要了解更多森貝伽實(shí)驗(yàn)代做服務(wù),請(qǐng)來電咨詢或點(diǎn)擊ELISA常見問題頁面。
三.森貝伽ELISA試劑盒組成: 產(chǎn)品組成 | 48孔配置 | 96孔配置 | Storage |
標(biāo)準(zhǔn)品:180ng/L | 0.5ml×1瓶 | 0.5ml×1瓶 | 2-8℃ |
標(biāo)準(zhǔn)品稀釋液 | 1.5ml×1瓶 | 1.5ml×1瓶 | 2-8℃ |
酶標(biāo)試劑 | 3ml×1瓶 | 6ml×1瓶 | 2-8℃ |
樣品稀釋液 | 3ml×1瓶 | 6ml×1瓶 | 2-8℃ |
顯色劑A液 | 3ml×1瓶 | 6ml×1瓶 | 2-8℃ |
顯色劑B液 | 3ml×1瓶 | 6ml×1瓶 | 2-8℃ |
終止液 | 3ml×1瓶 | 6ml×1瓶 | 2-8℃ |
濃縮洗滌液 | (20ml×20倍)×1瓶 | (20ml×30倍)×1瓶 | 2-8℃ |
酶標(biāo)包被板 | 1×48 | 1×96 | 2-8℃ |
封板膜 | 2片 | 2片 | RT |
密封袋 | 1個(gè) | 1個(gè) | RT |
說明書 | 1份 | 1份 | RT |
四.操作步驟: 五.注意事項(xiàng):1.試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開封后如未用完,板條應(yīng)裝入密封袋中保存。
2.濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果。
3.各步加樣均應(yīng)使用加樣器,并經(jīng)常校對(duì)其準(zhǔn)確性,以避免試驗(yàn)誤差。一次加樣時(shí)間最好控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排槍加樣。
4.請(qǐng)每次測定的同時(shí)做標(biāo)準(zhǔn)曲線,最好做復(fù)孔。如標(biāo)本中待測物質(zhì)含量過高(樣本OD值大于標(biāo)準(zhǔn)品孔孔的OD值),請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)(n倍)后再測定,計(jì)算時(shí)請(qǐng)最后乘以總稀釋倍數(shù)(×n×5)。
5.封板膜只限一次性使用,以避免交叉污染。
6.底物請(qǐng)避光保存。
7.嚴(yán)格按照說明書的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).
8.所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理。
9.本試劑不同批號(hào)組分不得混用。
10.如與英文說明書有異,以英文說明書為準(zhǔn)。
六.標(biāo)準(zhǔn)曲線的繪制方法:
可以采用各種繪圖軟件來繪制ELISA標(biāo)準(zhǔn)曲線,我公司以“Curve Exert1.3”軟件為例,繪制的ELISA標(biāo)準(zhǔn)曲線如下:
計(jì)算:以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的OD值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度;再乘以稀釋倍數(shù);或用標(biāo)準(zhǔn)物的濃度與OD值計(jì)算出標(biāo)準(zhǔn)曲線的直線回歸方程式,將樣品的OD值代入方程式,計(jì)算出樣品濃度,再乘以稀釋倍數(shù),即為樣品的實(shí)際濃度
七.特別聲明:南京森貝伽生物科技有限公司長期專業(yè)供應(yīng)各類科研產(chǎn)品,包括ELISA試劑盒、生物試劑、標(biāo)準(zhǔn)品對(duì)照品、培養(yǎng)基、抗體、染色液染色試劑盒等優(yōu)質(zhì)產(chǎn)品。森貝伽本著客戶至上的原則為客戶提供高質(zhì)量高品質(zhì)的產(chǎn)品,公司承諾如有質(zhì)量的問題免費(fèi)包退換(非人為因素造成的)。歡迎來電咨詢人N端中段骨鈣素(N-MID-OT)ELISA試劑盒最新報(bào)價(jià)、實(shí)驗(yàn)原理、產(chǎn)品用途及中英文說明書!
標(biāo)簽:人 ELISA試劑盒 人N端中段骨鈣素(N-MID-OT)
1、Haijun Liu,Shencun Fang,Wei Wang,etc.Macrophage-derived MCPIP1 mediates silica-induced pulmonary fibrosis via autophagy[J].Part Fibre Toxicol.2016:1-17.
作者單位:東南大學(xué)
使用產(chǎn)品:Human MCP-1, TNF-α, TGF-β ELISA kit
2、Juan Zhang,Meng Cao,Wenwen Yang,etc.Inhibition of Glucose-6-Phosphate Dehydrogenase Could Enhance 1,4-Benzoquinone-Induced Oxidative Damage in K562 Cells[J].Oxid Med Cell Longev.2016:1-11.
作者單位:東南大學(xué)
使用產(chǎn)品:Human NADP, NADPH ELISA Kit
3、Qian-NanDi,Wei-XinCao,RunXu,etc.Chronic low-dose exposure of nonylphenol alters energy homeostasis in the reproductive system of female rats[J].Toxicology and Applied Pharmacology.2018,1(348):67-75.
作者單位:東南大學(xué)
使用產(chǎn)品:Rat E2 ELISA Kit
4、Muhammad Noman Khan,Bing Wang,Jing Wei,etc.CXCR1/2 antagonism with CXCL8/Interleukin-8 analogue CXCL8(3-72)K11R/G31P restricts lung cancer growth by inhibiting tumor cell proliferation and suppressing angiogenesis[J].Oncotarget.2015,6(25): 21315-21327.
作者單位:國立清華大學(xué)(臺(tái)灣)
使用產(chǎn)品:Human CXCL1, CXCL6, CXCL8 ELISA kit
5、Cheng Yu Wang,Xiang Dan Li,Zhi Hong Hao,etc.Insulin-like growth factor-1 improves diabetic cardiomyopathy through antioxidative and anti-inflammatory processes along with modulation of Akt/GSK-3β signaling in rats[J].Korean J Physiol Pharmacol.2016,20(6): 613-619.
作者單位:延邊大學(xué)
使用產(chǎn)品:Rat IL-1β, TNF-a ELISA kit
6、Xiaoyue Shen,Wenkui Sun,Yi Shi,etc.Altered Viral Replication and Cell Responses by Inserting MicroRNA Recognition Element into PB1 in Pandemic Influenza A Virus (H1N1) 2009[J].Mediators Inflamm.2015:1-12.
作者單位:南京大學(xué)
使用產(chǎn)品:Human IL-6 ELISA kit
7、Chun‐Tao Lei,Hua Su,Chen Ye,etc.The classic signalling and trans-signalling of interleukin‐6 are both injurious in podocyte under high glucose exposure[J].J Cell Mol Med.2018,22(1):251-260.
作者單位:華中科技大學(xué)
使用產(chǎn)品:Human sIL‐6R, sgp130 ELISA kit
8、Jian’an Bai,He Na,Xiumei Hua,etc.A retrospective study of NENs and miR-224 promotes apoptosis of BON-1 cells by targeting PCSK9 inhibition[J].Oncotarget.2017,8(4): 6929-6939.
作者單位:南京醫(yī)科大學(xué)附屬逸夫醫(yī)院
使用產(chǎn)品:Human glucocorticoid ELISA kit
9、Zhan Zhang, Di Wang,Shanlei Qiao,etc.Metabolic and microbial signatures in rat hepatocellular carcinoma treated with caffeic acid and chlorogenic acid[J].Scientific reports.2017(7):1-10.
作者單位:南京醫(yī)科大學(xué)
使用產(chǎn)品:Rat LPS ELISA kit
10、Meng-Chen Lu,Jian-Ai Ji,Yong-Lin Jiang,etc.An inhibitor of the Keap1-Nrf2 protein-protein interaction protects NCM460 colonic cells and alleviates experimental colitis[J].Scientific reports. 2016(6):1-13.
作者單位:中國藥科大學(xué)
使用產(chǎn)品:Human IL-1β, IL-6, TNF-α, IFN-γELISA kit
11、Xiaotong Sun,Jing Wei,Yawei Tang,etc.Leptin-induced migration and angiogenesis in rheumatoid arthritis is mediated by reactive oxygen species[J].FEBS Open Bio.2017,7(12):1899-1908.
作者單位:大連醫(yī)科大學(xué)
使用產(chǎn)品:Human VEGF, IL‐1β, TNF-α ELISA kit
12、Geng Tian,Linlin Cheng,Xuewei Qi,etc.Transgenic Cotton Plants Expressing Double-stranded RNAs Target HMG-CoA Reductase (HMGR) Gene Inhibits the Growth, Development and Survival of Cotton Bollworms[J].Int J Biol Sci. 2015,11(11):1296-1305.
作者單位:華中農(nóng)業(yè)大學(xué)
使用產(chǎn)品:Plant HMGR ELISA kit
13、Siyuan Cui,Lili Men,Yu Li,etc.Selenoprotein S Attenuates Tumor Necrosis Factor-α-Induced Dysfunction in Endothelial Cells[J].Mediators Of Inflamm.2018:1-16.
作者單位:大連醫(yī)科大學(xué)附屬第一醫(yī)院
使用產(chǎn)品:Human ICAM-1, VCAM-1 ELISA kit
14、Peng-Tao Ren,Yuan Zhang,etc.Comparative investigation of the effects of specific antigen-sensitized DC-CIK and DC-CTL cells against B16 melanoma tumor cells[J].Mol Med Rep. 2017,15(4):1533-1538.
作者單位:河北醫(yī)科大學(xué)第二醫(yī)院
使用產(chǎn)品:Human CCL19, CCL22 ELISA kit
15、Ming Ma,Lian-mei Zhao,Xing-xiao Yang,etc.p-Hydroxylcinnamaldehyde induces the differentiation of oesophageal carcinoma cells via the cAMP-RhoA-MAPK signalling pathway[J].Scientific reports.2016(6):1-13.
作者單位:河北醫(yī)科大學(xué)第四醫(yī)院
使用產(chǎn)品:Human cAMP, CEA, SCC ELISA kit
16、Yucai Chen,Tianyi Yuan,Huifang Zhang,etc.Activation of Nrf2 Attenuates Pulmonary Vascular Remodeling via Inhibiting Endothelial-to-Mesenchymal Transition: an Insight from a Plant Polyphenol[J].Int J Biol Sci.2017,13(8):1067-1081.
作者單位:中國醫(yī)學(xué)科學(xué)院中國協(xié)和醫(yī)科大學(xué)藥物研究所
使用產(chǎn)品:Human MDA ELISA kit
17、Rao Sun,Zuoxia Zhang,Yishan Lei,etc.Hippocampal activation of microglia may underlie the shared neurobiology of comorbid posttraumatic stress disorder and chronic pain[J].Mol Pain. 2016(12):1-13.
作者單位:南京大學(xué)醫(yī)學(xué)院鼓樓醫(yī)院
使用產(chǎn)品:Human IL-1β, TNF-α ELISA kit
18、Yuanyuan Zhuang,Jiabao Liu,Pei Ma,etc.Tamarixinin A Alleviates Joint Destruction of Rheumatoid Arthritis by Blockade of MAPK and NF-κB Activation[J].Front Pharmacol.2017(8):1-11.
作者單位:北京協(xié)和醫(yī)學(xué)院
使用產(chǎn)品:Mouse CII antibody ELISA kit
19、Huawen Shi,Guangchao Yang,Tongsen Zheng,etc.A preliminary study of ALPPS procedure in a rat model[J].Scientific reports. 2015(5):1-12.
作者單位:哈爾濱醫(yī)科大學(xué)附屬第一醫(yī)院
使用產(chǎn)品:Rat NF-KB ELISA kit
20、Bo Qiu,Ming Gong,Qi-Ting He,etc.Controlled Release of Interleukin-1 Receptor Antagonist from Hyaluronic Acid-Chitosan Microspheres Attenuates Interleukin-1β-Induced Inflammation and Apoptosis in Chondrocytes[J].Biomed Res Int.2016:1-12.
作者單位:武漢大學(xué)人民醫(yī)院
使用產(chǎn)品:Rat GAG ELISA kit
21、Gairu Li,Yang Xiang,Jin Zhao,etc.Saccharum Alhagi polysaccharide-1 and -2 promote the immunocompetence of RAW264.7 macrophages in vitro[J].Exp Ther Med.2018,15(4): 3556-3562.
作者單位:新疆醫(yī)科大學(xué)
使用產(chǎn)品:Mouse IL-2, IL-1β, TNF-α ELISA kit
22、Yuanyuan Zhang,Minhui Sun,Yuwei Han,etc.The saponin DT-13 Attenuates Tumor Necrosis Factor-α-induced Vascular Inflammation Associated with Src/NF-кB/MAPK Pathway Modulation[J].Int J Biol Sci.2015,11(8):970-981.
作者單位:中國藥科大學(xué)
使用產(chǎn)品:Mouse ICAM-1 (sICAM-1), VCAM-1 (sVCAM-1) ELISA kit
23、Ying Huang,Shaofeng Wang,Chunya Zhang,etc.Experimental study of the anti-atherosclerotic effect of demethylzeylasteral[J].Exp Ther Med.2017,13(6): 2787-2792.
作者單位:上海市人民醫(yī)院
使用產(chǎn)品:Rabbit anti-oxidized LDL (ox-LDL) antibody ELISA kit
24、Lin Li,Ting Xiao,Fang Li,etc.Hydrogen sulfide reduced renal tissue fibrosis by regulating autophagy in diabetic rats[J].Mol Med Rep.2017,16(2): 1715-1722.
作者單位:華南大學(xué)第一附屬醫(yī)院
使用產(chǎn)品:Rat UP ELISA kit
25、Yong Zhang,Changyin Li,Hu Li,etc.miR-378 Activates the Pyruvate-PEP Futile Cycle and Enhances Lipolysis to Ameliorate Obesity in Mice[J].EBioMedicine.2016(5): 93-104.
作者單位:中國醫(yī)學(xué)科學(xué)院
使用產(chǎn)品:Mouse Insulin, FFA ELISA kit
26、Haitao Zou,Ruixue Su,Jing Ruan,etc.Toll-like receptor 3 pathway restricts Marek’s disease virus infection[J].Oncotarget.2017,8(41):70847-70853.
作者單位:揚(yáng)州大學(xué)
使用產(chǎn)品:Chicken IFN-β ELISA kit
27、Na Yang,Run-bin Sun,Xing-long Chen,etc.In vitro assessment of the glucose-lowering effects of berberrubine-9-O-β-D-glucuronide,an active metabolite of berberrubine[J].Acta Pharmacol Sin.2017,38(3): 351-361.
作者單位:中國藥科大學(xué)
使用產(chǎn)品:Rat α- glucosidase ELISA kit
28、Xiaoxiao Qian,Juan Tan,Ling Liu,etc.MicroRNA-134-5p promotes high glucose-induced podocyte apoptosis by targeting bcl-2[J].Am J Transl Res.2018,10(3): 989-997.
作者單位:南京醫(yī)科大學(xué)附屬第二醫(yī)院
使用產(chǎn)品:Mouse microalbuminuria ELISA Kit
29、Ruiping Fan,Yuwei Han,Han Han,etc.DT-13 ameliorates TNF-α-induced nitric oxide production in the endothelium in vivo and in vitro[J].Biochemical and Biophysical Research Communications.2018,1(495):1175-1181.
作者單位:中國藥科大學(xué)
使用產(chǎn)品:Mouse nitric oxide, IL-8, MCP-1, ROS, TNFR ELISA kit
30、Yu Li,Ning Hao,Suping Zou,etc.Immune Regulation of RAW264.7 Cells In Vitro by Flavonoids from Astragalus complanatus via Activating the NF-κB Signalling Pathway[J].Journal of Immunology Research.2018:1-9.
作者單位:安徽農(nóng)業(yè)大學(xué)
使用產(chǎn)品:Mouse IL-1β, IL-6, TNF-α, NO ELISA Kit
31、Xichun Wang,Shibin Feng,Nana Ding,etc.Anti-Inflammatory Effects of Berberine Hydrochloride in an LPS-Induced Murine Model of Mastitis[J].Evidence-Based Complementary and Alternative Medicine,2018:1-9.
作者單位:安徽農(nóng)業(yè)大學(xué)
使用產(chǎn)品:Mouse TNF-α, IL-6, and IL-1β ELISA Kit
32、JieChao,YanLv,JinChen,etc.SiO2-induced release of sVEGFRs from pulmonary macrophages[J].Respiratory Physiology & Neurobiology,2018(247):1-8.
作者單位:東南大學(xué)
使用產(chǎn)品:Human sVEGFR ELISA Kit
33、Meng-Chen Lu, Qiong Jiao,Tian Liu,etc.Discovery of a head-to-tail cyclic peptide as the Keap1-Nrf2 protein-protein interaction inhibitor with high cell potency[J].European Journal of Medicinal Chemistry.2018,1(143):1578-1589.
作者單位:中國藥科大學(xué)
使用產(chǎn)品:Mouse IL-1β, IL-18, TNF-α ELISA Kit
34、Zhengfeng Wu,Tianhong Gao,Ronglign Zhong,etc.Antihyperlipidaemic effect of triterpenic acid-enriched fraction from Cyclocarya paliurus leaves in hyperlipidaemic rats[J].Pharmaceutical Biology,2017,1(55):712-721.
作者單位:中國藥科大學(xué)
使用產(chǎn)品:Rat apoB48 ELISA Kit
35、Rao Sun,Zhibin Zhao,Jiying Feng,etc.Glucocorticoid-Potentiated Spinal Microglia Activation Contributes to Preoperative Anxiety-Induced Postoperative Hyperalgesia[J].Mol Neurobiol.2017,54(6):4316-4328.
作者單位:南京鼓樓醫(yī)院
使用產(chǎn)品: Rat CS, IL-1β, TNF-α ELISA Kit
36、Guoxiang You, Peifang Wang, Jun Hou,etc.Insights into the short-term effects of CeO2 nanoparticles on sludge dewatering and related mechanism[J].Water Research.2017,04(011):93-113.
作者單位:河海大學(xué)
使用產(chǎn)品:Plant LAP ELISA Kit
37、Peifang Wang, Guoxiang You, Jun Hou,etc.Responses of wastewater biofilms to chronic CeO2 nanoparticles exposure: Structural, physicochemical and microbial properties and potential mechanism[J].Water Research.2018:208-217.
作者單位:河海大學(xué)
使用產(chǎn)品:Plant AHL ELISA Kit
38、Yi Xu, Chao Wang, Jun Hou,etc.Strategies and relative mechanisms to attenuate the bioaccumulation and biotoxicity of ceria nanoparticles in wastewater biofilms[J].Bioresource Technology.2018:102-109.
作者單位:河海大學(xué)
使用產(chǎn)品:Plant ATP, DHA ELISA Kit
39、Junsheng Liu,Zhennan Gu,Wenwei Lu,etc.Multiple mechanisms applied by Lactobacillus pentosus AT6 to mute the lethal effects of Salmonella in a mouse model[J].Food Funct.2018, 23;9(5):2787-2795.
作者單位:江南大學(xué)
使用產(chǎn)品:Mouse CRP, LPS ELISA Kit